|
KMID : 0358819910180030390
|
|
Journal of Korean Society of Plastic and Reconstructive Surgeons
1991 Volume.18 No. 3 p.390 ~ p.406
|
|
|
STUDY ON THE GROWTH CHARACTERISTICS OF IN VITRO CULTURED HUMAN EPIDERMAL CELLS
|
|
|
|
|
Abstract
|
|
|
Skin autograph is an ideal procedure to resurface burn wounds of patients with extensive burns. However, since the availability of autogenous skin tissue is practically limited in such patients, many attempts were made by researchers to obtain expanded tissue or cell sheets from tiny skin fragments using various in vitro culture techniques.
Nevertheless, the effective means for such cultures have not been fully established.
The present study was undertaken to investigate the most effective procedures for in vitro cultivation of human epidermal cells using 113 skin fragments from 111 normal subjects. Growth characteristics of cultured cells as well as the effects of various biological and chemical agents on their growth were evaluated.
The results were summarized as follows
1. Treatment of minced skin fragments with 0.25% trypsin-0.1% ethylenediaminetetraacetic acid(EDTA) resulted in an active proliferation of epithelioid cells around the fragments,
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|